{"id":222,"date":"2018-11-15T15:19:57","date_gmt":"2018-11-15T15:19:57","guid":{"rendered":"https:\/\/www.deltafarmpress.com\/rice\/crispr-gene-editing-could-accelerate-plant-improvements"},"modified":"2018-12-17T16:33:53","modified_gmt":"2018-12-17T16:33:53","slug":"crspr-gene-editing-could-accelerate-plant-improvements","status":"publish","type":"post","link":"https:\/\/agrilife.org\/cgel\/2018\/11\/15\/crspr-gene-editing-could-accelerate-plant-improvements\/","title":{"rendered":"CRISPR Gene Editing Could Accelerate Plant Improvements"},"content":{"rendered":"
\n

When Dr. Tim Croughan was looking for rice that could be resistant to the herbicide imazethapyr, he tested thousands of mutant plants he developed in his laboratory at the LSU AgCenter\u2019s Rice Research Station in Crowley, La.<\/p>\n

After Croughan identified one resistant line in the early 1990s, it took a team of LSU researchers led by Dr. Steve Linscombe several more years to transfer the resistance into a Clearfield rice variety that could be grown commercially in the southern United States.<\/p>\n

The odds are that process could be replicated in a fraction of the time with gene editing using CRISPR Cas9 technology, according to Dr. Michael J. Thomson, professor in the Department of Soil and Crop Sciences at Texas A&M University and holder of the Henry M. Beachell Chair in international rice improvement.<\/p>\n

\u201cIf you look at what we\u2019ve traditionally done with traits such as Roundup Ready or glyphosate-tolerant cotton and soybeans, our tolerance was provided by a transgenic approach,\u201d said Thomson, speaking during a University of Arkansas System Division of Agriculture Food and Agribusiness Webinar (https:\/\/youtu.be\/6IQTKu42Los<\/a>).<\/p>\n

\u201cSo now there are ways to do the modification of essentially the endogenous genes (which originate from within an organism, tissue or cell) so that you can use CRISPR Cas9 in what could be a non-transgenic approach to this.\u201d<\/p>\n

CRISPR or Clustered Regularly Interspaced Short Palindromic Repeats technology was first described in a paper in 1987. It was first proposed as a potential tool for genome editing in a landmark paper written by Dr. Jennifer Doudna of the University of California, Berkeley, and Dr. Emmanuelle Charpentier from France in 2012.<\/p>\n

CRISPR and the CRISPR-associated protein 9 or Cas9, a protein found in the Streptococcus <\/em>bacterial immune system, cooperate with guide RNA and work like scissors, attacking the DNA of invading viruses and slicing it up. By \u201cknocking out\u201d selected genes, it can also help scientists better understand the function of those genes or allow other genes to more fully express themselves.<\/p>\n

See also: Researchers add gene editing to rice research toolbox<\/a><\/h4>\n

That would be a far cry from the process used to develop Clearfield rice, which is non-transgenic. \u201cIn the past, using mutation breeding, it was still quite a long process to find the perfect mutation \u2014 essentially it\u2019s a brute force method,\u201d said Thomson. \u201cYou\u2019re just making a lot of random mutations in the genome, and you\u2019re hoping that you can get the right one for your desired effect.\u201d<\/p>\n

With the acetolactate synthase inhibiting or ALS class of herbicides, for example, research has uncovered some of the reasons why weeds become resistant to those chemistries in field settings.<\/p>\n

\u201cIn this case, we can do a very targeted mutation,\u201d he said. \u201cWith those amino acid changes we have a lot of data on what provides that herbicide tolerance, and we can use that make those modifications in our materials for breeding lines.<\/p>\n

\u201cNow, this is a little tricky in that you don\u2019t want to overuse some of these herbicide tolerance genes because then, again, you\u2019ll have the tolerant weed problem. But this could be used as a way to pyramid multiple mechanisms or modes of action of herbicide tolerance into the same line so you have, then, many options when you\u2019re dealing with weeds.\u201d<\/p>\n

See also: Gene editing: Better crops, fewer regulations<\/a>?<\/h4>\n

In another recent publication, scientists described using biolistics to modify the amino acids for ALS resistance in corn, using a method called ribonucleoprotein or RNP delivery. \u201cWhat you see is that it does have the herbicide tolerance, provided by these specific amino acid changes.,\u201d Thomson said. \u201cBut what\u2019s interesting is that with RNP delivery, it\u2019s essentially delivery of CRISPR Cas9. In the end, there\u2019s no DNA that\u2019s integrated into the genome; it\u2019s essentially a DNA-free approach to gene editing. And it\u2019s also essentially non\u2011transgenic, as well.<\/p>\n

\u201cThis method uses a purified Cas9 protein with guide RNA mixture, and you make that complex in\u00a0vitro before you shoot it into the plant cells. You\u2019re starting with a Cas9 protein and the RNA complex. Then you introduce it using the gene gun, so biolistics can make that edit very quickly. It\u2019s a transient approach.\u201d<\/p>\n

After a week or two, the protein will be naturally degraded by the cells, but that\u2019s enough time for it to make the desired mutation in the target genome, Thomson says. \u201cIn this case, there\u2019s no DNA integration of any foreign DNA, and this would be one of the more straightforward ways to obtain a non-GMO product.\u201d<\/p>\n

Thomson says there are new products being developed by companies such as Calyxt, using gene editing techniques. Among those are high-oleic content soybean oil, high-fiber wheat, powdery mildew-resistant wheat, anti-browning mushrooms, disease-resistant citrus, fungus-resistant bananas and reduced-gluten what. Growers may also have new crops to grow, crops developed from wild plants domesticated by using CRISPR technology.<\/p>\n

Numerous legal and regulatory hurdles remain for the technology, although early indications are neither USDA nor the Food and Drug Administration are keen on regulating the products primarily derived through gene editing.<\/p>\n

Based on the early work by Doudna, the University of California, Berkely, has become involved in patent litigation with such coalitions as the Massachusetts Institute of Technology and the Broad Institute.<\/p>\n

\u201cThe medical field is the biggest area of application, but agriculture has gotten a lot of attention recently,\u201d said Thomson. \u201cA year ago DuPont Pioneer and the Broad Institute essentially joined forces to enable licensing of the technology for agriculture. It\u2019s provided some clarity in that groups know who to talk to if they want to license the technology.\u201d<\/p>\n

For more information on the University of Arkansas webinar series, visit https:\/\/bit.ly\/2qytJUu<\/a>.<\/p>\n<\/div>\n

Source: CRSPR Gene Editing Could Accelerate Plant Improvements<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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